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mouse embryonic fibroblast mef cells  (ATCC)


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    ATCC mouse embryonic fibroblast mef cells
    Mouse Embryonic Fibroblast Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 566 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblast+mef+cells/MEF/pm41811437-212-20-25
    Average 95 stars, based on 566 article reviews
    mouse embryonic fibroblast mef cells - by Bioz Stars, 2026-10
    95/100 stars

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    other:

    Article Title: Synergistic targeting of FASN and HMGCS1 by cerulenin enhances tumor cell ferroptosis sensitivity through rewiring lipid metabolism and blocking GPX4 biosynthesis.
    Article Snippet: Ferroptosis, an iron-dependent form of regulated cell death driven by lipid peroxidation, has emerged as a promising therapeutic target for cancer.. However, accumulating evidence indicates that tumor cells exhibit insensitivity to classic ferroptosis inducers.. In the present study, through high-throughput screening of a metabolite library, we identified cerulenin as a potent ferroptosis sensitizer.

    Article Title: Selenomethionine as a dual-mechanism ferroptosis inhibitor: selenium-supply-driven GPX4 biosynthesis beyond transsulfuration and reductive-capacity-mediated ROS scavenging independent of GPX4 activity
    Article Snippet: HT1080 cells, OS-RC-2, HepG2, H1299, A549, and mouse embryonic fibroblast (MEF) cells were obtained from the American Tissue Culture Collection (ATCC) and authenticated by the distributors.

    Article Title: Sustainable, scalable nanotechnology approach using filtrate from Raphanus sativus in combating multidrug-resistant pathogens and causing neglected tropical diseases
    Article Snippet: Mouse embryonic fibroblast (MEF) cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Article Title: Directed differentiation of bovine trophoblast stem cells: A useful in vitro model for placenta development.
    Article Snippet: Each blastocyst was placed in a separate well in a 12- well plate that was seeded with mitomycin C-tr eated mouse embryonic fibroblast (MEF) cells (ATCC, SCRC1008).

    Article Title: Selenomethionine as a dual-mechanism ferroptosis inhibitor: selenium-supply-driven GPX4 biosynthesis beyond transsulfuration and reductive-capacity-mediated ROS scavenging independent of GPX4 activity.
    Article Snippet: HT1080 cells, OS-RC-2, HepG2, H1299, A549, and mouse embryonic fibroblast (MEF) cells were obtained from the American Tissue Culture Collection (ATCC) and authenticated by the distributors.

    Article Title: The nucleocytoplasmic translocation of HINT1 regulates the maturation of cell density
    Article Snippet: Human skeletal muscle (hsSKM) and mouse embryonic Fibroblast (MEF) cells were purchased from ATCC.

    Modification:

    Article Title: Venezuelan Equine Encephalitis Virus Antagonizes the cGAS-STING Pathway.
    Article Snippet: Human microglial cells (HMC3) (ATCC, CRL-3304, Manassas, VA, USA) were maintained in Eagle’s Minimum Essential Medium with L-glutamine (EMEM) (Quality Biological, 112-018-101, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS) (Avantor, 97068-085, Radnor, PA, USA) and 1% penicillin/streptomycin (Pen-Strep) (Quality Biological, 120-095-721, Gaithersburg, MD, USA). .. Mouse embryonic fibroblast (MEF) cells (ATCC, CRL-2991, Manassas, VA, USA) and African green monkey kidney epithelial (Vero) cells (ATCC, CCL-81, Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, 11960-044, Grand Island, NY, USA) supplemented with 10% FBS, 1% L-glutamine (Corning, 25-005-Cl, Manassas, VA, USA) and 1% Pen-Strep. .. A plasmid containing the V5-capsid gene block (V5-C gene block) was ordered from Integrated DNA Technologies and transformed into Escherichia coli using the One ShotTM TOP10 kit (Invitrogen, C404010, Carlsbad, CA, USA).

    Article Title: Venezuelan Equine Encephalitis Virus Antagonizes the cGAS-STING Pathway
    Article Snippet: Human microglial cells (HMC3) (ATCC, CRL-3304, Manassas, VA, USA) were maintained in Eagle’s Minimum Essential Medium with L-glutamine (EMEM) (Quality Biological, 112-018-101, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS) (Avantor, 97068-085, Radnor, PA, USA) and 1% penicillin/streptomycin (Pen-Strep) (Quality Biological, 120-095-721, Gaithersburg, MD, USA). .. Mouse embryonic fibroblast (MEF) cells (ATCC, CRL-2991, Manassas, VA, USA) and African green monkey kidney epithelial (Vero) cells (ATCC, CCL-81, Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, 11960-044, Grand Island, NY, USA) supplemented with 10% FBS, 1% L-glutamine (Corning, 25-005-Cl, Manassas, VA, USA) and 1% Pen-Strep. .. A plasmid containing the V5-capsid gene block (V5-C gene block) was ordered from Integrated DNA Technologies and transformed into Escherichia coli using the One ShotTM TOP10 kit (Invitrogen, C404010, Carlsbad, CA, USA).



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    (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
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    (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic <t>fibroblast</t> (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.
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    (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic fibroblast (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.

    Journal: PLOS Pathogens

    Article Title: Nucleus softens during herpesvirus infection

    doi: 10.1371/journal.ppat.1013873

    Figure Lengend Snippet: (A) Cryo-soft X-ray (SXT) tomography ortho-slices of noninfected (NI) and infected mouse embryonic fibroblast (MEF) cells at 8 hpi. Molecular densities are presented in a continuous grey scale representing linear absorption coefficient values ranging from 0.1 to 0.4 μm -1 (calibration bar). Scale bars, 2 μm. (B) Cross-sections of 3D SXT reconstructions of a noninfected and an infected cell. The high-density region (heterochromatin) is shown in blue, the low-density region (euchromatin) in yellow, the viral replication compartment (VRC/euchromatin) area in green, and the cytoplasm in grey. See also . Quantitative analysis of linear absorption coefficient (LAC) of the (C) low-density and (D) high-density region in noninfected and infected cells at 4 and 8 hpi (n = 6). (E) Representative cross-sections of focused ion beam scanning electron microscopy (FIB-SEM) images of noninfected and infected MEF cells at 8 hpi. The viral capsid (arrows) and nuclear pore complexes (arrowheads) are shown. Scale bars, 0.5 µm. See also . (F) Reconstruction of chromatin fiber thickness and (G) chromatin density as a function of the distance from the nuclear envelope in noninfected and infected cells. The color scale in (F) indicates high and low chromatin thickness (yellow-blue), and in (G) high and low density (yellow-blue). The error bars show the standard deviation. Statistical significance was determined using Tukey’s test, and the significance values are denoted as * (p < 0.05). Nonsignificant differences (p ≥ 0.05) are not labeled.

    Article Snippet: Mouse embryonic fibroblast cells (MEF, ATCC CRL-2991) and African green monkey kidney cells (Vero, ATCC CCL-81) were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, L-glutamine, and penicillin-streptomycin (Gibco-Invitrogen) at 37 °C in the presence of 5% CO 2.

    Techniques: Tomography, Infection, Electron Microscopy, Standard Deviation, Labeling